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Effects of concentration and permeation time of cryoprotectants on larvae activity index in the larvae of the Pacific oyster Crassostrea gigas

Ki Tae Kim, Hae-Kyun Yoo, Saebom Sohn and Myung-Mo Nam

East Sea Fisheries Research Institute NIFS, Gangneung 25435, Korea

The Pacific oyster Crassostrea gigas larvae were exposed to 4 cryoprotectants, dimethyl sulfoxide (DMSO), ethylene glycol (EG), glycerol and methanol, each with 4 four concentrations (10, 20, 30, and 40%) for permeation. Detail concentration of experiment on larvae were exposed to 2 two cryoprotectants, DMSO and EG, each with 4 four concentrations 22, 24, 26 and 28%) for permeation. 26% dimethyl surfoxide (DMSO) for 30 min and 28% EG for 120 min were determined as appropriate CPA and equilibration time by CPA permeation experiments of trochophore larvae. 22% DMSO for 60 min and 26% EG for 60 min were determined as appropriate CPA and equilibration time by CPA permeation experiments of D-shaped larvae. Larval abnormalities, a sign of the chemical damage, were a representative phenotype which was higher at a higher concentration and longer duration of the chemicals.

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