Expressed sequence tag analysis of Meretrix lusoria (Veneridae) in Korea
Jung-Ha Kang, Ji Eun Jeong, Bong Seok Kim, Chel-Min An, Hyun-Sook Kang, Se-Won Kang, Hee Ju Hwang, Yeon Soo Han, Sung-Hwa Chae, Hyun-Sook Ko, Jun-Sang Lee and Yong Seok Lee
Biotechnology Research Division, NFRDI, Busan 619-705, Korea Department of Parasitology, College of Medicine and UHRC, Inje University, Busan 614-735, Korea Department of Agricultural Biology, Chonnam National University, Gwangju 500-757, Korea Researc

The importance of biological resources has been gradually increasing, and mollusks have been utilized as main fishery resources in terrestrial ecosystems. But little is known about genomic and transcript-xional analysis in mollusks. This is the first report on the transcript-xomic profile of Meretrix lusoria. In this study, we constructed cDNA library and determined 542 of distinct EST sequences composed of 284 singletons and 95 contigs. At first, we identified 180 of EST sequences that have significant hits on protein sequences of the exclusive Mollusks database through BLASTX program and 343 of EST sequences that have significant hits on NCBI NR database. We also found that 211 of putative sequences through local BLAST (blastx, E < e-10) search against KOG database were classified into 16 functional categories. Some kinds of immune response related genes encoding allograft inflammatory factor 1 (AIF-1), B-cell translocation gene 1 (BTG1), C-type lectin A, thioester-containing protein and 26S proteasome regulatory complex were identified. To determine phylogenetic relationship, we identified partial sequences of four genes (COX1, COX2, 12S rRNA and NADH dehydrogenase) that significantly matched with the mitochondrial genomes of 3 species-Ml (Meretrix lusoria), Mp (Meretrix petechialis) and Mm (Meretrix meretrix). As a result, we found that there was a little bit of a difference between sequences of Korean isolates and other known isolates. This study will be useful to develop breeding technology and might also be helpful to establish a classification system.

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