전자저널 > 전자저널 > Comparison of Cryopreservation Effects on D-shaped Larvae of Arkshell, Scapharca broughtonii (Pelecypoda: Acidae) by the Kinds of Additive and the Concentrations of Cryoprotectant

Comparison of Cryopreservation Effects on D-shaped Larvae of Arkshell, Scapharca broughtonii (Pelecypoda: Acidae) by the Kinds of Additive and the Concentrations of Cryoprotectant

Pil Gue Jo, Youn Hee Choi, Kyoung Ho Kang, Kang Hee Kho, Chang-Soon Go, Byong-Hak Kim, Han Kyu Lim, Cheol-Young Choi and Young Jin Chang

Department of Aquaculture, Pukyong National University, Busan 608-737, Korea

This study was performed to find out the optimal kind of additive and concentration of ethylene glycol (EG) as the cryoprotectant on the D-shaped larvae of arkshell, Scapharca broughtonii. The larvae in straws was carried in the programed freezer set up at 0℃, frozen to -12℃by the freezing rate of -1℃/min, held for 10 minutes, seeded at -12℃, finally refrozen to -3 5℃by the freezing rate of -1℃/min and then directly plunged into liquid nitrogen. The survival of larvae frozen in 1.0 M and 2.0 M ethylene glycol added with 0.5 M sucrose were high (50.5 ± 1.3% and 51.9 ± 1.7%, respectively) in the early D-shaped larvae cryopreserved in 1 M and 2 M ethylene glycol diluted with 0.2 M and 0.5 M fructose, glucose and sucrose. In the late D-shaped larvae were cryopreserved according to five concentrations of ethylene glycol added with 0.5 M sucrose, the survival of larvae frozen in 2.0 M ethylene glycol was the highest as 51.9 ± 1.7%. The morphological differences in cells between unfrozen and frozen-thawed D-shaped larvae were not found by the scanning and transmission electron microscopy.

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