The updated PANM database version 6.0 includes approximately 1.3 times more amino acid sequences compared to its previous version. Although it contains only about 3.6% of the sequences compared to the commonly used NCBI-nr database for annotation, PANM 6.0 was specifically constructed using amino acid sequences of genes from Arthropoda, Nematoda, and Mollusca. This allows for reduced annotation time and improved accuracy for genes, genomes, or transcript-xome sequences of species belonging to these three phyla. Like previous versions, PANM database version 6 (http://panm.sch.ac.kr/) freely accessible to all researchers and is expected to be useful for researchers studying invertebrates in the future.