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Comparative transcriptome analysis of the response of Pacific abalone (Haliotis discus hannai) after adaptive or acute heat stress condition
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Eun-Ha Shin, Eun Hee Park, Eun-Soo Noh, Young-Ok Kim and Bo-Hye Nam |
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Biotechnology Research Division, National Institute of Fisheries Science, Busan 46083, Korea
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The climate change causes the mass mortality of farmed fish and shellfish. Therefore, it is important to comprehend clearly the physical and molecular mechanisms of abalone under heat stress condition. In order to find genes involved in biological response to heat stress, we have performed sequencing of transcript-xome in two different group of the pacific abalone (Haliotis discus hannai), the breeding line group and the general aquaculture group, after adaptive treatment (18¡ÆC ¡æ 20¡ÆC ¡æ 25¡ÆC ¡æ 30¡ÆC) or acute treatment (18¡ÆC ¡æ 30¡ÆC for 0.5hr, 1hr, 3hr or 6hr). Tissue-specific differential expression genes (DEGs) were identified with 52 genes in gonad, 28 in hemocytes, 36 in gills, 31 in muscle and 33 in mantle under different heat shock conditions. There was no significantly differentially expressed pattern of transcript-xome in the tested tissues between two groups, except the DEGs from the gonad tissues. Among them, the expression patterns of the XBP1, OS9, VCP, SNRNP70, MGAT2, UBA5, DST, ST13, YOD1 and ERP44 genes in the gonad tissue of the breeding line group showed significant differences from those of all tissues in the general aquaculture group. The present study provides a foundation for understanding the molecular mechanism of heat stress tolerance in abalone. Further functional studies should be performed on the heat stress-related genes identified in this study, which can contribute to the molecular breeding for heat stress tolerance in abalone.
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37-2-5-61-68.pdf (919.0K), Down : 164, 2021-08-26 13:58:07
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