Genetic Variation of Wild and Hatchery Populations of the Korean Ark Shell, Scapharca broughtonii Assessed by Microsatellite Markers

Young Ju Jee, Woo Jin Kim, Byung Hak Kim, Soon Gyu Byun and Kee Chae Cho
Biotechnology Research Division, National Fisheries Research and Development Institute, Gijang-gun, Busan 619-705, Korea Southwest Sea Fisheries Research Institute, National Fisheries Research and Development Institute, Hwayang-myun, Yeosu-city, Jeon Nam
The genetic variation of Ark Shell, Scapharca broughtonii black was estimated using six polymorphic microsatellite (MS) loci in 443 individuals collected from five populations in Korea. The mean numbers of alleles per locus in five populations were 10-28. The mean number of alleles per locus in Jinhae Hatchery (JHH) population showed the least value as 15.5, but that in Gangjin (GJ) population showed the most value as 20.3. The mean expected heterozygosity in Saryangdo (SR) population showed the least value as 0.817, but that in Gangjin (GJ) population showed the most value as 0.831. In Jinhae hatchery(JHH) population, the mean expected heterozygosity was 0.822, there was no significant difference from those of wild population. The FST values in Gangjin (GJ) population showed significant difference from those of the other populations, which revealed Gangjin (GJ) population is genetically different from the other populations. The FST values among Jinhae Hatchery (JHH) population, Jinhae (JH) population and Saryangdo (SR) population showed  lower values than the others, which implies there was a gene flow among these three populations. The FST value and genetic distance between Jinhae (JH) population and Saryangdo (SR) population showed the least value as 0.0001 and 0.0386, indicating that these two populations were genetically the same.     
  
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