전자저널 > 전자저널 > Prismatic shell repairs by hemoctyes in the extrapallial fluid of the Pacific Oyster, Crassostrea gigas

Prismatic shell repairs by hemoctyes in the extrapallial fluid of the Pacific Oyster, Crassostrea gigas

Sang-Man Cho and Woo-Geon Jeong

Department of Aquaculture and Aquatic Sciences, Kunsan National University, 1170 Miryong, Kusan, Jeonbuk 573-160, Korea Department of Marine biology and Aquaculture & Institute of Marine Industrial Science, Gyeongsang National University, 445 Inpyeong,

To understand the role of hemocytes in the shell repair process, a hole was drilled in the right valves of the Pacific oyster, Crassostrea gigas, and the repair process was observed. Histological observations suggested that the exterior surface of the shell was repaired by aggregated hemocytes. The nuclei of the hemocytes were cleary stained in the regenerated shell while appearing fragmented after calcification at the 7th day. Globular calcium granules were genegenerated from the hemocytic monolyer after 6 days of incubation which were morphologically and chemically identical with those from prismatic shell. Our finding suggested that the repaired prismatic shell was composed by aggregated hemocytes and that their endogenous calcium component might support the nucleation of calcium biomineralization during shell repair.

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