전자저널 > 전자저널 > Species Identification and Genetic Structure of Octopus minorfrom Korea and China on the Basis of Partial Sequences ofMitochondrial Cytochrome Oxidase I

Species Identification and Genetic Structure of Octopus minor
from Korea and China on the Basis of Partial Sequences of
Mitochondrial Cytochrome Oxidase I

Jung-Ha Kang, Ki Hwan Yu, Sang-Kyu Kim, Jung-Yeon Park, Bong-Seok Kim and Chel-Min An

Biotechnology Research Division, NFRDI, Busan 619-705, Korea

The nucleotide sequences of the mitochondrial cytochrome oxidase subunit 1 (CO1) gene of octopus groups collected from Muan, Taean, Yesu, Jeju in Korea and Youngsung, Daeryen in China were analyzed for the identification of species and populations. Six haplotypes were identified from the analyzed 60 individuals. All of the individuals (N = 10) from Jeju showed the A haplotype which was not observed from other groups, and could be classified as a distinct group. The analyzed groups could form two separate clade in MEGA4 analysis. The individuals from Muan, Taean, Yesu in Korea and Daeryen in China form a clase and the others from Jeju in Korea and Youngsung in China formed the other clade. The analysis of relationship among the groups showed the same results. Individuals belong to the group A Muan, Taean, Yesu and Daeryen) showed closer relationship than individuals belong to the group B (Jeju and Youngsung). Although the CO1 universal primers used in this study was useful as a marker for species identification among Octopus, analysis of population was limited because of few variations in the partial sequences of CO1 analyzed in this study. However, it was possible to show the limited gene flow among the groups which is resulted from the spatial separation and differences in their habitats.

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