전자저널 > 전자저널 > Comparison of Nucleotide Sequences of 28S rDNA from Two Viviparid Snail Species in Korea: Cipangopaludina chinensis malleata and C. japonica

Comparison of Nucleotide Sequences of 28S rDNA from Two Viviparid Snail Species in Korea: Cipangopaludina chinensis malleata and C. japonica

Gab-Man Park, Younghun Jung, Jae-Jin Kim and Pyung-Rim Chung

Research Institute of Natural Sciences, Pai Chai University, Taejon

The Korean rice-field snails belonging to family Viviparidae have regarded as an important freshwater molluscs, because they are edible as food source in Korea and play a role as the intermediate hosts of some trematode parasites. However, their taxonomic problems have not been clearly solved yet. Kwon et al.(1993) recognized two viviparid species in Korea, Cipangopaludina chinensis malleata and C. japonica. C. chinensis malleata distribute nation-widely everywhere in Korea, such as rivers, streams, ponds and rice-fields, but the geographical distribution of C. japonica is much restricted only in the southern part of Korea (Park et al., 1997). In recent years, genetic variations of the organisms have been investigated for phylogenetic analysis using the nuclear genomes. The nuclear ribosomal RNA gene (rDNA) repeat unit has regions evolving at varying rates, and also has been used extensively to study genetic variations and phylogeny at a number of taxonomic levels. Polymerase chain reaction-restriction fragment length polymor phism (PCR-RFLP) techniques have been developed for molecular genetic identification and examination of the genetic diversity in populations (Bowditch et al., 1993; Liu and Mitton, 1993). Genetic variations of the 28S rRNA gene detected by PCR-RFLP were made as markers of genetic identity (Matsuura et al., 1992; Fujino et al., 1995). In this study, PCR-RFLP analysis and nucleotide sequences of 28S rDNA between two Korean viviparid species were done to compare their genetic distances. The results obtained in this study were also compared with the nucleotide data from other molluscan species to determine the percentages of homology.

v132p1.pdf (505.2K), Down : 102, 2008-03-24 13:03:00