전자저널 > 전자저널 > Cryopreservation of Pacific Oyster, Crassostrea gigas Sperm

Cryopreservation of Pacific Oyster, Crassostrea gigas Sperm

Mi Seon Park, Byung Hwa Min, Jung Jun Park, Hyun Jeong Lim, Jeong-In Myeong and Min Hwan Jeong

Aquaculture Management Division, National Fisheries Research and Development Institute, Busan 619-902, Korea West Sea Fisheries Research Institute, National Fisheries Research and Development Institute, Incheon 400-420, Korea

This study aims to find out a suitable cryoprotective agent (CPA) for cryopreservation and its optimum concentration in order to conduct planned artificial seed production of Pacific oyster, Crassostrea gigas and to preserve superior sperm. For this, we tried to understand toxicity and the effect of cryopreservation by CPA type and concentrations first and then looked into cell damage of the sperm after thawing. Toxicity analysis on the sperm of Pacific oyster according to different CPA and immersion time shows that dimethyl sulfoxide (DMSO) comes first when it comes to survival rate and mobility followed by ethylene glycol (EG), glycerol and methanol. To identify the optimum CPA and its level, filtered seawater was used as a diluent before cryopreservation for 30 days. As a result, cryopreserved sperm of Pacific oyster with 15% of DMSO showed the highest survival rate and activation. Also, we observed the cryopreserved and thawed sperm with Scanning electron micrographs by CPAs and concentrations. Consequently, DSMO showed the lowest cell damage followed by EG, methanol, glycerol and the level was 15, 20, 10, 5% respectively. In a nutshell, it is proven that the optimum CPA and its level is 15% of DMSO.

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